The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified. Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide. The contrast between stained cells and background facilitates image analysis. The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture.
生物安全等級
1
生長培養基
DMEM＋10%FBS＋1%P/S
推薦傳代比例
1:4-1:8
推薦換液頻率
2~3次/周
凍存條件
凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮
培養條件
氣相：空氣，95%；CO2，5%溫度：37℃
致瘤性
Yes, forms tumors in the brains of CD Fischer 344 rats
