The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia. The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy.
生物安全等級
1
生長培養基
MEM＋10% FBS＋1%P/S
推薦傳代比例
1:3-1:6
推薦換液頻率
2~3次/周
倍增時間
33.6 hours, at 48th passage (PubMed=2993532); 34 hours (ATCC)
凍存條件
凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮
培養條件
氣相：空氣，95%；CO2，5%溫度：37℃
致瘤性
Yes, in nude mice (The cells form serially transplantable intercranial and subcutaneous tumors.)
