產品信息 | 名稱 | NCCIT (人畸胎癌細胞) (STR鑒定正確) | | 別稱 | NCC-IT | | 種屬 | 人 | | 年齡（性別） | 男；24歲 | | 組織來源 | 多能性胚胎癌；畸胎癌 | | 生長特性 | 貼壁細胞 | | 細胞形態 | 上皮細胞樣 | | 背景描述 | NCCIT was established by Shinichi Teshima (National Cancer Institute, Tokyo, Japan) in 1985 from a mediastinal mixed germ cell tumor. | | 生物安全等級 | 1 | | 生長培養基 | RPMI-1640＋10% FBS＋1%P/S | | 推薦傳代比例 | 1:4-1:8 | | 推薦換液頻率 | 2~3次/周 | | 凍存條件 | 凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮 | | 培養條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ | | 致瘤性 | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. | | 保藏機構 | ATCC; CRL-2073 |
STR位點信息 | Amelogenin | X | | CSF1PO | 10,12 | | D2S1338 | 18,19 | | D3S1358 | 16 | | D5S818 | 10,13 | | D7S820 | 10 | | D8S1179 | 10,15 | | D13S317 | 11 | | D16S539 | 9,12 | | D18S51 | 13,14 | | D19S433 | 13,15 |
| D21S11 | 29,32.2 | | FGA | 22,26 | | PentaD | 10,12 | | PentaE | 5,14 | | TH01 | 7,9 | | TPOX | 8 | | vWA | 14,18 | | D1S1656 | 15,17 | | D6S1043 | 14 | | D12S391 | 18,22 |
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