LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes' C mucin secreting cecal tumor located at the Bauhin valve.
生物安全等級
1
生長培養基
RPMI-1640＋10% FBS＋1%P/S
推薦傳代比例
1:3-1:5
推薦換液頻率
2~3次/周
倍增時間
20 hours (PubMed=25984343)
凍存條件
凍存液：55% 基礎培養基+40%FBS+5%DMSO溫度：液氮
培養條件
氣相：空氣，95%；CO2，5%溫度：37℃
致瘤性
Yes, forms tumors in nude mice
抗原表達情況
carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive
